Luminol Chemiluminescence Test for Blood

Luminol Chemiluminescence Test for Blood The luminol chemiluminescence reaction is responsible for the glow of lightsticks. The reaction is used by criminalists to detect traces of blood at crime scenes. In this test, luminol powder (C8H7O3N3) is mixed with hydrogen peroxide (H2O2) and a hydroxide (e.g., KOH) in a spray bottle. The luminol solution is sprayed where blood might be found. The iron from the hemoglobin in the blood serves as a catalyst for the chemiluminescence reaction that causes luminol to glow, so a blue glow is produced when the solution is sprayed where there is blood. Only a tiny amount of iron is required to catalyze the reaction. The blue glow lasts for about 30 seconds before it fades, which is enough time to take photographs of the areas so they can be investigated more thoroughly. Heres how you can detect blood yourself or demonstrate how to do it: Luminol Materials luminol stock solution (2 g luminol 15 g potassium hydroxide 250 mL water)3% hydrogen peroxide in water (common over-the-counter concentration)potassium ferricyanide or a sterile blood lancet and sterile alcohol pad Performing the Test or Demonstration In a clear test tube or cup, mix 10 ml of the luminol solution and 10 ml of the peroxide solution.You can activate the glow either by adding ~0.1 g of potassium ferricyanide to the solution or with a drop of blood. The blood must be on the alcohol pad. The forensic test is for dried or latent blood, so the reaction between the alcohol and fresh blood is necessary. Notes About the Luminol Test In addition to iron and iron compounds, other substances can catalyze the luminol reaction. Copper and its compounds, horseradish, and bleach also cause the solution to glow. So, you could substitute any of these materials for the drop of blood or potassium ferricyanide in the demonstration. Similarly, the presence of these chemicals at a crime scene affects testing for blood. If a crime scene was washed in bleach, for example, the whole area would glow when sprayed with luminol, making it necessary to use a different test to find traces of blood.If you are doing the reaction as a chemiluminescence demonstration, you can kick the display up a notch by dissolving the potassium ferricyanide in the peroxide solution and using a fractionating column or glass spiral to react the solutions rather than a test tube. You could pour a small amount of fluorescein in the bottom of a flask, pour the potassium ferricyanide solution through the spiral into the flask, and (in a darkened room) finish by adding the luminol solution. The spiral will glow blue as it passes through the column, but the glow will change to bright green once the luminol touches the fluorescein in the flask. Dont drink the luminol solution. Dont get it on your skin or in your eyes. If you prepare a spray bottle of luminol solution to check for traces of blood, keep in mind the solution might be damaging to some surfaces. Thats not a big factor at a crime scene, but its something to keep in mind at home or in class. Dont spray upholstery or clothing or people.The quantities of chemicals yield a super-bright demonstration, but you  can use much less luminol (~50 mg) and still achieve enough luminescence for a demonstration or for crime work. How the Luminol Test Works The iron in the hemoglobin found in blood catalyzes an oxidation reaction in which the luminol gains oxygen atoms while losing nitrogen and hydrogen. This produces a compound called 3-aminophthalate. The electrons in the 3-aminophthalate are in an excited state. Blue light is emitted as energy is released when the electrons return to the ground state. Learn More The luminol test is only one method used to detect blood. The Kastle-Meyer test is a chemical test used to detect extremely small quantities of blood. If you have leftover potassium ferricyanide, you can use it to grow naturally red crystals. Although the chemical name sounds scary, with the cyanide word in it, its actually a very safe chemical to use.

Europe between Wars 1919-1939 and European Crisis Assignment

Europe between Wars 1919-1939 and European Crisis - Assignment Example The Weimar Republic had to face and go through a severe economic fall in the first years of the decade because of the debt and the reparations caused by the War and the Treaty of Versailles, which culminated in 1923 with the devaluation of the Mark (the currency). Communism attracted many followers after the October Revolution and the Russian Civil War, and so communists (Bolsheviks) adopted a policy of mixed economies in the period between 1921 and 1928. Fascist movement as a response and a solution for the prevention of the communism spread in many countries in Europe, with Germany having the leading role. This period was believed to be the third period of anxiety for Europe (the previous two that could be compared in intensity with this one being the one of the ancient world and the Reformation). Totalitarianism as a concept in political theory arose in the XX century. Although there are slight differences and variations among totalitarian regimes, the chief characteristics of totalitarianism are: a) Totalitarian regime encompasses the whole of the society, and authoritarian government, as a variation focuses only on governance. For a regime to be considered totalitarian, government and society must be closely intertwined and there is only one political party; b) Totalitarianism holds strict government control of the media which is to be used only as a means of propaganda; c) There is no personal liberty in cultural, political and artistic expression among the people; d) People must be utterly devoted to the state and must never criticize the government or any of its segments as they and their whole families may "disappear" or end up in prison as "political" prisoners. e) Nazism and Fascism are considered totalitarian regimes of the right, while Stalinism is the totalitarianism of the left.

Marketing and International Retailing Essay Example | Topics and Well Written Essays - 4500 words

Marketing and International Retailing - Essay Example 64), and that certain iue relating to corporate ocial reponibility are placed within the ame trategic context a, for example, pricing and continuity of upply. In the year ahead, retail trategit are likely to focu increaed attention on the election of appropriate partner organization (Wrigley, 1994, pp. 6-7; Lowe and Wrigley, 1996; Morganoky, 1997, p. 369; Martin et al., 1998, p. 14) and put in place appropriate leaderhip model to enure that interaction between individual throughout the partnerhip manifet itelf in buine deciion which in turn reult in a utainable competitive advantage being achieved and maintained. Thi mean that manager need to: A regard cutomer retention in particular, taff in each of the partner organization will in future need to be more pro-active in promoting the concept of cutomer relationhip management, which i underpinned by a clearly defined et of organizational value. A well crafted relationhip marketing trategy will help to enure that long-term, quality-baed relationhip are developed with taff in partner organization throughout the marketing channel (Grant, 1991, p. 45; Juttner and Peck, 1998). The adoption of thi approach will link marketing policy to human reource management policy, and attention will be focued on the co-ordination and flow of information among taff, between function and facilitate, and on the promotion of inter-organizational interaction (Cepede, 1995, pp. 251-2; Platt and Yeung, 2000). Furthermore, retail marketing planner and manager need to adopt a reflective approach to marketing deciion-making, and thi mean engaging more with marketing academic in order that a critical evaluation can be made of marketing policy and trategy related deciion. The reult hould be the development of marketing knowledge. New inight generated can be converted into new theoretical approache (Burton, 2005, p. 11). The outcome hould prove beneficial by advancing marketing theory, linking it to practice, and explaining development in current area of tudy uch a cutomer relationhip management (Baker, 2003). That aid, it i a quetion worthy of cloe conideration whether or not all thi can be achieved without a more holitic appreciation of what marketing intelligence repreent. An anwer will be offered during the coure of the paper. Firt, however, attention will be turned to what contitute a cutomer-driven international trategy, to the matter of developing long-term relationhip, and to the building of long-term partnerhip arrangement. Thee are conidered to be the key factor to be conidered when deviing a retailing trategy. Cutomer-Driven International trategie The focu on international trategy i not intended to exclude conideration of dometic trategy, but rather reflect the reality of twenty-firt century retailing and the focu of many of the other author whoe work i reviewed. That increaingly dynamic buine environment force manager to implement change, poibly through incremental adaptation and, when neceary, experimentation. Marketing manager therefore need to be aware of how intelligence gathered from the external and internal

GA AEMT Drug Cards Essay Example for Free

GA AEMT Drug Cards Essay ACTION: Reverses Hypoxia, Increases arterial oxygen, Increases hemoglobin saturation, Increases tissue oxygenation Indications: Hypoxia or anticipated hypoxia, or in any medical or trauma patient to improve respiratory efficiency. Contraindications: There are no contraindications to oxygen therapy. Dose: Route: Oral DOSAGE: Adult: 1-6 LPM via NC; 10-15 LPM via NRB; 15 LPM via BVM Pediatric: 1-6 LPM via NC; 10-15 LPM via NRB; 15 LPM via BVM Common side effects: Dry or bloody nose, skin irritation from the nasal cannula or face mask Precautions/major interactions: Chronic obstructive pulmonary disease (COPD) and very prolonged administration of high concentrations in the newborn. Drug: Oral Glucose Generic Glutose, Insta-Glucose Class of medication: Monosaccharide Carbohydrate ACTION: After absorption from GI tract, glucose is distributed in the tissues and provides a prompt increase in circulating blood sugar Onset: 10 min Indications: Patients with altered mental status and known history of diabetes mellitus Contraindications: Unconsciousness; known diabetic who has not taken insulin for days; patient who is unable to swallow Dose: Route: Oral DOSAGE/ROUTE:Adult: One tube or packet; repeat based on BGL Pediatric: One tube or packet; repeat based on BGL – minimum age 3 yrs Common side effects: Nausea, may be aspirated by the patient without a gag reflex Precautions/major interactions Assure patient has control of swallowing and airway. Drug: Glucagon Generic: GlucaGen Class of medication: Hormone, antihypoglycemic ACTION: Glucagon is a protein secreted by pancreatic cells that causes a breakdown of stored glycogen into glucose and inhibits the synthesis of glycogen from glucose. Indications: Hypoglycemia without IV access. Contraindications: Hypersensitivity to glucagon or protein compounds. Dose: Route: Intramuscular Adult:1-2mg IM, repeat in 15 minutes if BGL

Julius Caesar: Brutus Vs. Cassius Essay -- essays research papers

Contrasting Characters In Shakespeare’s The Tragedy of Julius Caesar, Brutus and Cassius are contrasting characters. They differ in the way they perceive Antony as a threat to the assassination plot, their dominance in personality, and their moral fiber. In Julius Caesar, Brutus is the more naà ¯ve, dominant and noble character, while Cassius is the more perceptive, submissive, and manipulative person. Brutus and Cassius are very different in the way they perceive Antony. Brutus is very trusting and naà ¯ve when he judges Antony. When the subject of killing Antony comes up among the conspirators, Brutus underestimates how dangerous Antony could be and says, â€Å"For Antony is but a limb of Caesar†(2.1.178). This statement means Brutus does not think it is necessary to kill Antony and he thinks that without Caesar, Antony is worthless. Another incident where Brutus misjudges Antony is when he allows Antony to speak at Caesar’s funeral. Brutus trusts that Antony will not say anything bad about the conspirators or him: â€Å"What Antony shall speak I will protest/ He speaks by leave and by permission, / And that we are contented Caesar shall/ Have all true rites and lawful ceremonies. / It shall advantage us more than do us wrong†(3.1.263-268). Brutus actually thinks that by letting Antony speak, the conspirators and he will have a better situation for themselve s because it will make their plot seem honorable. Cassius, on the other hand, is a very perceptive person; he sees how dangerous Antony can be. He notices that Antony is clever and that he might not be trustworthy. When Brutus suggests that they shouldn’t kill Antony, Cassius says if Antony outlives Caesar, â€Å"[They] shall find of [Mark Antony]/ a shrewd contriver† (2.1.170-171). When Brutus gives Antony the right to speak at Caesar’s funeral, Cassius pulls Brutus aside and says, †You know not what you do. Do/ not consent/ That Antony speak in [Caesar’s] funeral. / Know you how much the people may be moved/ by that which [Antony] will utter†(3.1.255-259)? Cassius sees that Antony is a powerful speaker and that if Antony speaks the people will side with him. This shows that Cassius has a much better idea of how dangerous Antony is. Although Cassius is correct on how dangerous Antony really is, Brutus’ ideas are used because Brutus is the more dominant character. In the play Cassius is the more passive character and ... ...proves Cassius is jealous and fears that Caesar will get to become king. He initiates an assassination plot and persuades Brutus to help him. After Cassius comments on how noble Brutus is, he talks about seducing Brutus into allying with him. To show that he says, â€Å"Therefore it is meet/ That noble minds keep ever with their likes; / For who so firm that cannot be seduced†(1.2.322-324). The word seduced itself means that Cassius is planning to trick Brutus into helping him. Again, Cassius shows his mischievous nature when he says how he is going to write false letters to Brutus and throw them in his window: â€Å"In several hands in at his windows throw, / As if they came from several citizens, / Writings, all tending to the great opinion that Rome holds of his name†¦Ã¢â‚¬  (1.2.227-230). This shows that Cassius is clever but not trustworthy. These examples prove that Brutus and Cassius differ between being noble and honorable, or conniving and mischievous. Brutus and Cassius have different ways of perceiving people, different personalities, and different values. They contradict each other in these three important ways, but together they play an important part in Shakespeare’s Julius Caesar.

Gaming the Console Response

2121 – Writing for MIT Dr. Tim Freeborn January 31, 2012 Gaming the Console – Rhetorical Essay In Linda Bernstein’s informative article â€Å"Gaming the Console† she presents both sides to the ongoing debate about the possible consequences associated with playing video games. Some experts have concluded that gaming leads to negative outcomes such as an increase in violent behavior, or a decrease in academic and social skills. On the other hand, there is a belief that some video games can have positive educational and physical benefits.Bernstein touches on all of these aspects of gaming and leaves the ultimate decision up to the reader to form his or her own opinion on the matter. Throughout the article, there are three images displayed that effectively support the text and help develop the arguments presented. The initial picture that is splashed across the entire first page of the article seems to represent the type of teen who exhibits the potential for increased aggression as a result of playing video games.The photo depicts a male teenager with spiked hair, dark eyes, pierced ear, and stubble on his lip and chin. These characteristics are typically indicative of a more rebellious or aggressive individual. His concentration is evident in his wide-eyed expression and his grimacing face with his tongue in his mouth. He holds the console in a tight grip as shown by the redness of his skin around his thumbs, which indicates his level of intensity. All of these details compliment the notion that video games may be unknowingly impacting areas of the brain associated with aggression.Professor Craig A. Anderson of Iowa State University describes this as follows: â€Å"The effects aren’t huge or immediately noticeable by a game player, but they tend to increase over time† (Bernstein 14). The player may not feel more aggressive after playing a game, but there may be evidence to suggest the effect is still there none the less. The next image in sequence works well in conjunction with the text and seems to support the argument that gaming can have positive benefits if used appropriately. For instance, in the article eighteen-year-old David A. xpresses, â€Å"It’s a fun way of being with your friends when [you are] home relaxing. [It is] competitive and fun, but I only do it when I have extra time† (Bernstein 14). This opinion appears to be shared by the two male and two female teens shown in the picture. They appear to be having fun while playing the game and getting along. Although they have a look of concentration on their faces, the fact that they are all leaning in the same direction could indicate they are playing towards a common goal, and thus encouraging teamwork.It shows boys and girls playing together which illustrates that gaming can have a social and inclusive benefit, and is not simply for socially awkward children. This image can support the notion that if gaming is not performe d at the exclusion of other things such as schoolwork and sports, it can be a fun pastime. The last image Bernstein integrates into her article could be perceived as support for either side of the debate over the impact of video games. The presentation of a boy with his headset on and console in the air with a huge smile indicates again a positive image of gaming.On the other hand, the fact that he is wearing headphones may signify that he is a serious gamer who spends a lot of time gaming at the exclusion of other educational activities. This picture is rather ambiguous because depending on whether he is part of the 8% teen gamers who are addicted or 92% who are not addicted it can have a very different meaning (Bernstein 14). Overall, this image can be interpreted to support whatever argument you are rooting for by the end of the article.Bernstein is successful in presenting useful images that enhance the text while still being fair and showing all views discussed throughout the a rticle. The first image portrays the aggressive nature of gaming, the second illustrates the social aspect of gaming and the third image is left up for interpretation by the viewer. Overall, the article displays a balanced perspective complimented with appropriate corresponding images that support Bernstein’s thesis. Works Cited Bernstein, Linda. â€Å"Gaming the Console. † Current Health Teens. † March 2012.

The Case Ban On Tobacco Ads And Talks About The Conflict...

This essay is a case study analysis that uses the case Ban on Tobacco Ads and talks about the conflict of interests between tobacco producers and the Government of India. In Feb 2001 the Government of India published the news on tabling a bill that bans Tobacco companies on advertising cigarettes and sponsoring the sports and other cultural events. (1) There are several arguments that support the Government’s decision: 1. Protection of people’s health. As World Health Organization states that the 3 million deaths in 1990 occurred as a result of smoking and in 1998 it raised to 4.023 million. 2. Preventing the children aged 14-24 years from being smokers. According to internal document from R J Reynolds (1998) the bright advertising of the cigarettes that had a camel’s picture on the packing was attractive to children and stimulated teenage smoking. 3. Contributions of cigarettes to the budget of India is 0.14% versus the health costs which account for 0.21%. 4. Provision of employment by the tobacco industry is an illusion because those who will not be employed in the tobacco field will be employed somewhere else and will be more labor intensive. 5. Complete bans on the tobacco products give a better decrease in tobacco consumption. This is evident in the investigation of UK’s Department of Health (1992) which found out that the banning of advertising resulted in fall of smoking. (2) Opposed to the Government there are several arguments from Tobacco companies thatShow MoreRelatedCase Studies67624 Words   |  271 PagesCase Studies C-1 INTRODUCTION Preparing an effective case analysis C-3 CASE 1 CASE 2 CASE 3 CASE 4 CASE 5 CASE 6 CASE 7 ABB in China, 1998 C-16 Ansett Airlines and Air New Zealand: A flight to oblivion? 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